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size exclusion chromatography  (Bio-Rad)


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    Bio-Rad size exclusion chromatography
    Size Exclusion Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 507 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/size exclusion chromatography/product/Bio-Rad
    Average 96 stars, based on 507 article reviews
    size exclusion chromatography - by Bioz Stars, 2026-02
    96/100 stars

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    size exclusion chromatography sec chromatograms  (Agilent technologies)
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    Agilent technologies size exclusion chromatography sec chromatograms
    Biophysical characterization of PA2706 (black symbols) and PA0932 (red symbols) (A) Far-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 4 μM protein (monomer concentration). (B) Near-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 60 μM protein (monomer concentration). (C) Oligomeric state <t>determination.</t> <t>Size-exclusion</t> <t>chromatography</t> <t>(SEC)</t> chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors. The proteins were analyzed at a concentration of 1 mg/mL. Data shown are a representative run of two independent experiments. (D) Thermal denaturation collected at 195 nm on solutions containing 4 μM protein in 20 mM potassium phosphate buffer, pH 7.0. Lines through data points are the fitting to with T m = 60.0 ± 0.1 °C for PA2706 and T m = 74.6 ± 0.2 °C for PA0932. Each value is the average of two independent experiments ± standard deviation.
    Size Exclusion Chromatography Sec Chromatograms, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/size exclusion chromatography sec chromatograms/product/Agilent technologies
    Average 96 stars, based on 1 article reviews
    size exclusion chromatography sec chromatograms - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier
    size exclusion chromatography  (JASCO Inc)
    94
    JASCO Inc size exclusion chromatography
    Biophysical characterization of PA2706 (black symbols) and PA0932 (red symbols) (A) Far-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 4 μM protein (monomer concentration). (B) Near-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 60 μM protein (monomer concentration). (C) Oligomeric state <t>determination.</t> <t>Size-exclusion</t> <t>chromatography</t> <t>(SEC)</t> chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors. The proteins were analyzed at a concentration of 1 mg/mL. Data shown are a representative run of two independent experiments. (D) Thermal denaturation collected at 195 nm on solutions containing 4 μM protein in 20 mM potassium phosphate buffer, pH 7.0. Lines through data points are the fitting to with T m = 60.0 ± 0.1 °C for PA2706 and T m = 74.6 ± 0.2 °C for PA0932. Each value is the average of two independent experiments ± standard deviation.
    Size Exclusion Chromatography, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/size exclusion chromatography/product/JASCO Inc
    Average 94 stars, based on 1 article reviews
    size exclusion chromatography - by Bioz Stars, 2026-02
    94/100 stars
    		  Buy from Supplier
    size exclusion chromatography  (Bio-Rad)
    96
    Bio-Rad size exclusion chromatography
    Biophysical characterization of PA2706 (black symbols) and PA0932 (red symbols) (A) Far-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 4 μM protein (monomer concentration). (B) Near-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 60 μM protein (monomer concentration). (C) Oligomeric state <t>determination.</t> <t>Size-exclusion</t> <t>chromatography</t> <t>(SEC)</t> chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors. The proteins were analyzed at a concentration of 1 mg/mL. Data shown are a representative run of two independent experiments. (D) Thermal denaturation collected at 195 nm on solutions containing 4 μM protein in 20 mM potassium phosphate buffer, pH 7.0. Lines through data points are the fitting to with T m = 60.0 ± 0.1 °C for PA2706 and T m = 74.6 ± 0.2 °C for PA0932. Each value is the average of two independent experiments ± standard deviation.
    Size Exclusion Chromatography, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/size exclusion chromatography/product/Bio-Rad
    Average 96 stars, based on 1 article reviews
    size exclusion chromatography - by Bioz Stars, 2026-02
    96/100 stars
    		  Buy from Supplier

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    Biophysical characterization of PA2706 (black symbols) and PA0932 (red symbols) (A) Far-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 4 μM protein (monomer concentration). (B) Near-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 60 μM protein (monomer concentration). (C) Oligomeric state determination. Size-exclusion chromatography (SEC) chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors. The proteins were analyzed at a concentration of 1 mg/mL. Data shown are a representative run of two independent experiments. (D) Thermal denaturation collected at 195 nm on solutions containing 4 μM protein in 20 mM potassium phosphate buffer, pH 7.0. Lines through data points are the fitting to with T m = 60.0 ± 0.1 °C for PA2706 and T m = 74.6 ± 0.2 °C for PA0932. Each value is the average of two independent experiments ± standard deviation.

    Journal: iScience

    Article Title: De novo cysteine biosynthesis in Pseudomonas aeruginosa : Characterization of the two main cysteine synthase isoforms

    doi: 10.1016/j.isci.2025.114304

    Figure Lengend Snippet: Biophysical characterization of PA2706 (black symbols) and PA0932 (red symbols) (A) Far-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 4 μM protein (monomer concentration). (B) Near-UV circular dichroism spectra were collected in a 20 mM potassium phosphate buffer, pH 7.0, at 20 °C on solutions containing 60 μM protein (monomer concentration). (C) Oligomeric state determination. Size-exclusion chromatography (SEC) chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors. The proteins were analyzed at a concentration of 1 mg/mL. Data shown are a representative run of two independent experiments. (D) Thermal denaturation collected at 195 nm on solutions containing 4 μM protein in 20 mM potassium phosphate buffer, pH 7.0. Lines through data points are the fitting to with T m = 60.0 ± 0.1 °C for PA2706 and T m = 74.6 ± 0.2 °C for PA0932. Each value is the average of two independent experiments ± standard deviation.

    Article Snippet: Size-exclusion chromatography (SEC) chromatograms obtained from an Agilent AdvanceBio 300 Å column coupled to UV-vis and dual-angle light scattering detectors.

    Techniques: Circular Dichroism, Concentration Assay, Size-exclusion Chromatography, Standard Deviation